inverted laser scanning confocal microscope ti a1plus Search Results


99
Nikon a1plus inverted confocal microscope
A1plus Inverted Confocal Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a1plus inverted confocal microscope - by Bioz Stars, 2026-08
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99
Nikon nikon tie inverted microscope
The images were taken by confocal <t>microscope</t> every 2 min over 30 min. Representative images of T cell-bilayer interface taken at the end of the observation period are shown: mature synapse ( a ), TCR/CD3 focal synapse ( b ), kinapse ( c ) and multifocal synapse ( d ). IRM images show contacts of CD8 T cells with the bilayer surface as dark area on light background. Histograms depict the fluorescent intensity profiles along the diagonal white lines in overlay images. Distance is measured in pixels. Blue: ICAM-1, green: anti-CD3, red: IRM. Scale bars: 5 μm. Representative kymographs show temporal changes in spatial position of ICAM-1 (blue) and anti-CD3 antibodies (green): mature synapse ( e ), TCR/CD3 focal interface ( f ), kinapse ( g ) and multifocal synapse ( h ). White oval indicates the position of the cell/bilayer interface at the initial contact of T cell with a bilayer surface, and time axis indicates temporal changes in the spatial position of ICAM-1 and CD3 locations. i Variations in adhesion area of CD8 T cells establishing different synaptic interfaces. The adhesion areas were determined from IRM images. For each donor group, the results of representative experiment are shown. The dots on the graphs present individual cells that form mature synapses, TCR/CD3 focal interfaces, kinapses, and multifocal synapses; HIV−: n = 30, 25, 10, 11; HIV+: n = 22, 10, 10, 11; HIV+ ART: n = 52, 18, 14, 15 cells, respectively. Means with SDs are indicated. Adjusted p values were calculated by ordinary one-way ANOVA with Tukey multiply comparison test, and the values are displayed on the top of the graphs. Data ( a – i ) represent one out of three independent experiments for HIV- donor group and one out of two independent experiments for each group of infected donors.
Nikon Tie Inverted Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
nikon tie inverted microscope - by Bioz Stars, 2026-08
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99
Nikon ti2 inverted microscope
The images were taken by confocal <t>microscope</t> every 2 min over 30 min. Representative images of T cell-bilayer interface taken at the end of the observation period are shown: mature synapse ( a ), TCR/CD3 focal synapse ( b ), kinapse ( c ) and multifocal synapse ( d ). IRM images show contacts of CD8 T cells with the bilayer surface as dark area on light background. Histograms depict the fluorescent intensity profiles along the diagonal white lines in overlay images. Distance is measured in pixels. Blue: ICAM-1, green: anti-CD3, red: IRM. Scale bars: 5 μm. Representative kymographs show temporal changes in spatial position of ICAM-1 (blue) and anti-CD3 antibodies (green): mature synapse ( e ), TCR/CD3 focal interface ( f ), kinapse ( g ) and multifocal synapse ( h ). White oval indicates the position of the cell/bilayer interface at the initial contact of T cell with a bilayer surface, and time axis indicates temporal changes in the spatial position of ICAM-1 and CD3 locations. i Variations in adhesion area of CD8 T cells establishing different synaptic interfaces. The adhesion areas were determined from IRM images. For each donor group, the results of representative experiment are shown. The dots on the graphs present individual cells that form mature synapses, TCR/CD3 focal interfaces, kinapses, and multifocal synapses; HIV−: n = 30, 25, 10, 11; HIV+: n = 22, 10, 10, 11; HIV+ ART: n = 52, 18, 14, 15 cells, respectively. Means with SDs are indicated. Adjusted p values were calculated by ordinary one-way ANOVA with Tukey multiply comparison test, and the values are displayed on the top of the graphs. Data ( a – i ) represent one out of three independent experiments for HIV- donor group and one out of two independent experiments for each group of infected donors.
Ti2 Inverted Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
ti2 inverted microscope - by Bioz Stars, 2026-08
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99
Yokogawa Electric csu-w1
The images were taken by confocal <t>microscope</t> every 2 min over 30 min. Representative images of T cell-bilayer interface taken at the end of the observation period are shown: mature synapse ( a ), TCR/CD3 focal synapse ( b ), kinapse ( c ) and multifocal synapse ( d ). IRM images show contacts of CD8 T cells with the bilayer surface as dark area on light background. Histograms depict the fluorescent intensity profiles along the diagonal white lines in overlay images. Distance is measured in pixels. Blue: ICAM-1, green: anti-CD3, red: IRM. Scale bars: 5 μm. Representative kymographs show temporal changes in spatial position of ICAM-1 (blue) and anti-CD3 antibodies (green): mature synapse ( e ), TCR/CD3 focal interface ( f ), kinapse ( g ) and multifocal synapse ( h ). White oval indicates the position of the cell/bilayer interface at the initial contact of T cell with a bilayer surface, and time axis indicates temporal changes in the spatial position of ICAM-1 and CD3 locations. i Variations in adhesion area of CD8 T cells establishing different synaptic interfaces. The adhesion areas were determined from IRM images. For each donor group, the results of representative experiment are shown. The dots on the graphs present individual cells that form mature synapses, TCR/CD3 focal interfaces, kinapses, and multifocal synapses; HIV−: n = 30, 25, 10, 11; HIV+: n = 22, 10, 10, 11; HIV+ ART: n = 52, 18, 14, 15 cells, respectively. Means with SDs are indicated. Adjusted p values were calculated by ordinary one-way ANOVA with Tukey multiply comparison test, and the values are displayed on the top of the graphs. Data ( a – i ) represent one out of three independent experiments for HIV- donor group and one out of two independent experiments for each group of infected donors.
Csu W1, supplied by Yokogawa Electric, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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99
Olympus vs120 high throughput fluorescence microscope
The images were taken by confocal <t>microscope</t> every 2 min over 30 min. Representative images of T cell-bilayer interface taken at the end of the observation period are shown: mature synapse ( a ), TCR/CD3 focal synapse ( b ), kinapse ( c ) and multifocal synapse ( d ). IRM images show contacts of CD8 T cells with the bilayer surface as dark area on light background. Histograms depict the fluorescent intensity profiles along the diagonal white lines in overlay images. Distance is measured in pixels. Blue: ICAM-1, green: anti-CD3, red: IRM. Scale bars: 5 μm. Representative kymographs show temporal changes in spatial position of ICAM-1 (blue) and anti-CD3 antibodies (green): mature synapse ( e ), TCR/CD3 focal interface ( f ), kinapse ( g ) and multifocal synapse ( h ). White oval indicates the position of the cell/bilayer interface at the initial contact of T cell with a bilayer surface, and time axis indicates temporal changes in the spatial position of ICAM-1 and CD3 locations. i Variations in adhesion area of CD8 T cells establishing different synaptic interfaces. The adhesion areas were determined from IRM images. For each donor group, the results of representative experiment are shown. The dots on the graphs present individual cells that form mature synapses, TCR/CD3 focal interfaces, kinapses, and multifocal synapses; HIV−: n = 30, 25, 10, 11; HIV+: n = 22, 10, 10, 11; HIV+ ART: n = 52, 18, 14, 15 cells, respectively. Means with SDs are indicated. Adjusted p values were calculated by ordinary one-way ANOVA with Tukey multiply comparison test, and the values are displayed on the top of the graphs. Data ( a – i ) represent one out of three independent experiments for HIV- donor group and one out of two independent experiments for each group of infected donors.
Vs120 High Throughput Fluorescence Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vs120 high throughput fluorescence microscope - by Bioz Stars, 2026-08
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99
Nikon nikon elements software
The images were taken by confocal <t>microscope</t> every 2 min over 30 min. Representative images of T cell-bilayer interface taken at the end of the observation period are shown: mature synapse ( a ), TCR/CD3 focal synapse ( b ), kinapse ( c ) and multifocal synapse ( d ). IRM images show contacts of CD8 T cells with the bilayer surface as dark area on light background. Histograms depict the fluorescent intensity profiles along the diagonal white lines in overlay images. Distance is measured in pixels. Blue: ICAM-1, green: anti-CD3, red: IRM. Scale bars: 5 μm. Representative kymographs show temporal changes in spatial position of ICAM-1 (blue) and anti-CD3 antibodies (green): mature synapse ( e ), TCR/CD3 focal interface ( f ), kinapse ( g ) and multifocal synapse ( h ). White oval indicates the position of the cell/bilayer interface at the initial contact of T cell with a bilayer surface, and time axis indicates temporal changes in the spatial position of ICAM-1 and CD3 locations. i Variations in adhesion area of CD8 T cells establishing different synaptic interfaces. The adhesion areas were determined from IRM images. For each donor group, the results of representative experiment are shown. The dots on the graphs present individual cells that form mature synapses, TCR/CD3 focal interfaces, kinapses, and multifocal synapses; HIV−: n = 30, 25, 10, 11; HIV+: n = 22, 10, 10, 11; HIV+ ART: n = 52, 18, 14, 15 cells, respectively. Means with SDs are indicated. Adjusted p values were calculated by ordinary one-way ANOVA with Tukey multiply comparison test, and the values are displayed on the top of the graphs. Data ( a – i ) represent one out of three independent experiments for HIV- donor group and one out of two independent experiments for each group of infected donors.
Nikon Elements Software, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
nikon elements software - by Bioz Stars, 2026-08
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99
Yokogawa Electric csu10 spinning disk confocal head
The images were taken by confocal <t>microscope</t> every 2 min over 30 min. Representative images of T cell-bilayer interface taken at the end of the observation period are shown: mature synapse ( a ), TCR/CD3 focal synapse ( b ), kinapse ( c ) and multifocal synapse ( d ). IRM images show contacts of CD8 T cells with the bilayer surface as dark area on light background. Histograms depict the fluorescent intensity profiles along the diagonal white lines in overlay images. Distance is measured in pixels. Blue: ICAM-1, green: anti-CD3, red: IRM. Scale bars: 5 μm. Representative kymographs show temporal changes in spatial position of ICAM-1 (blue) and anti-CD3 antibodies (green): mature synapse ( e ), TCR/CD3 focal interface ( f ), kinapse ( g ) and multifocal synapse ( h ). White oval indicates the position of the cell/bilayer interface at the initial contact of T cell with a bilayer surface, and time axis indicates temporal changes in the spatial position of ICAM-1 and CD3 locations. i Variations in adhesion area of CD8 T cells establishing different synaptic interfaces. The adhesion areas were determined from IRM images. For each donor group, the results of representative experiment are shown. The dots on the graphs present individual cells that form mature synapses, TCR/CD3 focal interfaces, kinapses, and multifocal synapses; HIV−: n = 30, 25, 10, 11; HIV+: n = 22, 10, 10, 11; HIV+ ART: n = 52, 18, 14, 15 cells, respectively. Means with SDs are indicated. Adjusted p values were calculated by ordinary one-way ANOVA with Tukey multiply comparison test, and the values are displayed on the top of the graphs. Data ( a – i ) represent one out of three independent experiments for HIV- donor group and one out of two independent experiments for each group of infected donors.
Csu10 Spinning Disk Confocal Head, supplied by Yokogawa Electric, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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csu10 spinning disk confocal head - by Bioz Stars, 2026-08
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86
Lumencor Inc sola light engine
The images were taken by confocal <t>microscope</t> every 2 min over 30 min. Representative images of T cell-bilayer interface taken at the end of the observation period are shown: mature synapse ( a ), TCR/CD3 focal synapse ( b ), kinapse ( c ) and multifocal synapse ( d ). IRM images show contacts of CD8 T cells with the bilayer surface as dark area on light background. Histograms depict the fluorescent intensity profiles along the diagonal white lines in overlay images. Distance is measured in pixels. Blue: ICAM-1, green: anti-CD3, red: IRM. Scale bars: 5 μm. Representative kymographs show temporal changes in spatial position of ICAM-1 (blue) and anti-CD3 antibodies (green): mature synapse ( e ), TCR/CD3 focal interface ( f ), kinapse ( g ) and multifocal synapse ( h ). White oval indicates the position of the cell/bilayer interface at the initial contact of T cell with a bilayer surface, and time axis indicates temporal changes in the spatial position of ICAM-1 and CD3 locations. i Variations in adhesion area of CD8 T cells establishing different synaptic interfaces. The adhesion areas were determined from IRM images. For each donor group, the results of representative experiment are shown. The dots on the graphs present individual cells that form mature synapses, TCR/CD3 focal interfaces, kinapses, and multifocal synapses; HIV−: n = 30, 25, 10, 11; HIV+: n = 22, 10, 10, 11; HIV+ ART: n = 52, 18, 14, 15 cells, respectively. Means with SDs are indicated. Adjusted p values were calculated by ordinary one-way ANOVA with Tukey multiply comparison test, and the values are displayed on the top of the graphs. Data ( a – i ) represent one out of three independent experiments for HIV- donor group and one out of two independent experiments for each group of infected donors.
Sola Light Engine, supplied by Lumencor Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
sola light engine - by Bioz Stars, 2026-08
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98
Nikon general defocusing particle tracking velocimetry gdptv
The images were taken by confocal <t>microscope</t> every 2 min over 30 min. Representative images of T cell-bilayer interface taken at the end of the observation period are shown: mature synapse ( a ), TCR/CD3 focal synapse ( b ), kinapse ( c ) and multifocal synapse ( d ). IRM images show contacts of CD8 T cells with the bilayer surface as dark area on light background. Histograms depict the fluorescent intensity profiles along the diagonal white lines in overlay images. Distance is measured in pixels. Blue: ICAM-1, green: anti-CD3, red: IRM. Scale bars: 5 μm. Representative kymographs show temporal changes in spatial position of ICAM-1 (blue) and anti-CD3 antibodies (green): mature synapse ( e ), TCR/CD3 focal interface ( f ), kinapse ( g ) and multifocal synapse ( h ). White oval indicates the position of the cell/bilayer interface at the initial contact of T cell with a bilayer surface, and time axis indicates temporal changes in the spatial position of ICAM-1 and CD3 locations. i Variations in adhesion area of CD8 T cells establishing different synaptic interfaces. The adhesion areas were determined from IRM images. For each donor group, the results of representative experiment are shown. The dots on the graphs present individual cells that form mature synapses, TCR/CD3 focal interfaces, kinapses, and multifocal synapses; HIV−: n = 30, 25, 10, 11; HIV+: n = 22, 10, 10, 11; HIV+ ART: n = 52, 18, 14, 15 cells, respectively. Means with SDs are indicated. Adjusted p values were calculated by ordinary one-way ANOVA with Tukey multiply comparison test, and the values are displayed on the top of the graphs. Data ( a – i ) represent one out of three independent experiments for HIV- donor group and one out of two independent experiments for each group of infected donors.
General Defocusing Particle Tracking Velocimetry Gdptv, supplied by Nikon, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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general defocusing particle tracking velocimetry gdptv - by Bioz Stars, 2026-08
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Image Search Results


The images were taken by confocal microscope every 2 min over 30 min. Representative images of T cell-bilayer interface taken at the end of the observation period are shown: mature synapse ( a ), TCR/CD3 focal synapse ( b ), kinapse ( c ) and multifocal synapse ( d ). IRM images show contacts of CD8 T cells with the bilayer surface as dark area on light background. Histograms depict the fluorescent intensity profiles along the diagonal white lines in overlay images. Distance is measured in pixels. Blue: ICAM-1, green: anti-CD3, red: IRM. Scale bars: 5 μm. Representative kymographs show temporal changes in spatial position of ICAM-1 (blue) and anti-CD3 antibodies (green): mature synapse ( e ), TCR/CD3 focal interface ( f ), kinapse ( g ) and multifocal synapse ( h ). White oval indicates the position of the cell/bilayer interface at the initial contact of T cell with a bilayer surface, and time axis indicates temporal changes in the spatial position of ICAM-1 and CD3 locations. i Variations in adhesion area of CD8 T cells establishing different synaptic interfaces. The adhesion areas were determined from IRM images. For each donor group, the results of representative experiment are shown. The dots on the graphs present individual cells that form mature synapses, TCR/CD3 focal interfaces, kinapses, and multifocal synapses; HIV−: n = 30, 25, 10, 11; HIV+: n = 22, 10, 10, 11; HIV+ ART: n = 52, 18, 14, 15 cells, respectively. Means with SDs are indicated. Adjusted p values were calculated by ordinary one-way ANOVA with Tukey multiply comparison test, and the values are displayed on the top of the graphs. Data ( a – i ) represent one out of three independent experiments for HIV- donor group and one out of two independent experiments for each group of infected donors.

Journal: Nature Communications

Article Title: The immune synapses reveal aberrant functions of CD8 T cells during chronic HIV infection

doi: 10.1038/s41467-022-34157-0

Figure Lengend Snippet: The images were taken by confocal microscope every 2 min over 30 min. Representative images of T cell-bilayer interface taken at the end of the observation period are shown: mature synapse ( a ), TCR/CD3 focal synapse ( b ), kinapse ( c ) and multifocal synapse ( d ). IRM images show contacts of CD8 T cells with the bilayer surface as dark area on light background. Histograms depict the fluorescent intensity profiles along the diagonal white lines in overlay images. Distance is measured in pixels. Blue: ICAM-1, green: anti-CD3, red: IRM. Scale bars: 5 μm. Representative kymographs show temporal changes in spatial position of ICAM-1 (blue) and anti-CD3 antibodies (green): mature synapse ( e ), TCR/CD3 focal interface ( f ), kinapse ( g ) and multifocal synapse ( h ). White oval indicates the position of the cell/bilayer interface at the initial contact of T cell with a bilayer surface, and time axis indicates temporal changes in the spatial position of ICAM-1 and CD3 locations. i Variations in adhesion area of CD8 T cells establishing different synaptic interfaces. The adhesion areas were determined from IRM images. For each donor group, the results of representative experiment are shown. The dots on the graphs present individual cells that form mature synapses, TCR/CD3 focal interfaces, kinapses, and multifocal synapses; HIV−: n = 30, 25, 10, 11; HIV+: n = 22, 10, 10, 11; HIV+ ART: n = 52, 18, 14, 15 cells, respectively. Means with SDs are indicated. Adjusted p values were calculated by ordinary one-way ANOVA with Tukey multiply comparison test, and the values are displayed on the top of the graphs. Data ( a – i ) represent one out of three independent experiments for HIV- donor group and one out of two independent experiments for each group of infected donors.

Article Snippet: Confocal fluorescent microscopy was carried out on scanning laser Nikon A1R + confocal microscope system equipped with Nikon TiE inverted microscope, Nikon A1plus camera, and four lasers with excitation lines at 405, 488, 561, and 640 nm.

Techniques: Microscopy, Comparison, Infection